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[WGBS] 不同脑神经元细胞的single cell DNA methylome(灌水帖)

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发表于 2017-10-13 21:56:47 | 显示全部楼层 |阅读模式
生物学的文章发表在2017年8月的Science杂志上, 英文摘要如下:
The mammalian brain contains diverse neuronal types, yet we lack single-cell epigenomic assays that are able to identify and characterize them. DNA methylation is a stable epigenetic mark that distinguishes cell types and marks regulatory elements. We generated >6000 methylomes from single neuronal nuclei and used them to identify 16 mouse and 21 human neuronal subpopulations in the frontal cortex. CG and non-CG methylation exhibited cell type–specific distributions, and we identified regulatory elements with differential methylation across neuron types. Methylation signatures identified a layer 6 excitatory neuron subtype and a unique human parvalbumin-expressing inhibitory neuron subtype. We observed stronger cross-species conservation of regulatory elements in inhibitory neurons than in excitatory neurons. Single-nucleus methylomes expand the atlas of brain cell types and identify regulatory elements that drive conserved brain cell diversity.


single cell bisulphite-sequencing 的实验方法发表在今年的Nature Protocols杂志上:
http://www.nature.com/nprot/journal/v12/n3/full/nprot.2016.187.html?WT.feed_name=subjects_molecular-biology





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