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iPSCs跟cancer cell lines and animal models.比较

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发表于 2017-10-26 11:50:01 | 显示全部楼层 |阅读模式
The use of iPSCs presents both advantages and disadvantages compared to traditional approaches such as the use of cancer cell lines and animal models.

First, iPSCs are species-specific and individual-specific, and thus cancer-causing mutations can be studied in the genomic context of the cancer patient. However, due to the stochastic nature of reprogramming and the resultant epigenetic variability, discerning whether the phenotype stems from individual clonal variability or from the general pathological mechanism may be difficult.

Secondly, iPSCs are renewable and scalable systems that allow high-throughput screening, making them especially desirable platforms for therapeutic drug screening and toxicological studies. Their pluripotency allows them to be differentiated into diverse cell types. Although genetically quite stable, iPSCs are nonetheless cell lines and may still accumulate undesirable mutations during prolonged propagation in culture, possibly undermining their full potential for cell-based therapies.

Thirdly, cancer-cell-derived iPSCs may be re-differentiated toward susceptible and resistant lineages, allowing us to study how specific oncogenic mutations impose the tumor phenotype on a particular cell lineage and/or developmental state. They may be used to study the interaction of specific oncogenic mutations with different cell types and their association with specific developmental states and cellular niches. The disadvantage is that the signaling pathways for re-creating developmental processes and fully differentiated cell types are still not well understood. Also, systemic processes cannot be fully modeled in vitro.



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 楼主| 发表于 2017-10-26 11:50:06 | 显示全部楼层
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 楼主| 发表于 2017-10-26 11:52:39 | 显示全部楼层
Human iPSCs have absolute advantages over human embryonic stem cells (ESCs) and animal models in disease modeling, drug screening, and cell replacement therapy. Since Takahashi and Yamanaka first described in 2007 that iPSCs can be generated from human adult somatic cells by retroviral transduction of the four transcription factors, Oct3/4, Sox2, Klf4, and c-Myc, disease specific iPSC lines have sprung up worldwide like bamboo shoots after a spring rain, making iPSC one of the hottest and fastest moving topics in modern science.
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