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以前用RNAi技术,现在改为CRISPR

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发表于 2018-3-29 11:55:08 | 显示全部楼层 |阅读模式
Previously, this was typically conducted using RNA interference (RNAi) in the form of short interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries ([url=]Boutros and Ahringer, 2008[/url], [url=]Luo et al., 2009[/url], [url=]Schlabach et al., 2008[/url], [url=]Silva et al., 2008[/url], [url=]Zuber et al., 2011[/url]).

Such screens have made important contributions to biology, but their success has been moderated by the varying efficiencies of siRNAs/shRNAs for the stringent and specific suppression of target genes required for genome-wide studies ([url=]Boutros and Ahringer, 2008[/url]).


CRISPR-Cas9-based functional genomics may be able to overcome such limitations and, therefore, hold great promise in re-shaping cell-essentiality screens. In cancer research, such screens can be applied to identify genetic vulnerabilities of cancer cells that can be used to develop new anti-cancer treatments.



来自于文章: http://www.cell.com/cell-reports/fulltext/S2211-1247(16)31335-3



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