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[assembly] megahit 组装报错Cannot identify read library type,如何解决?

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发表于 2019-2-22 08:54:23 | 显示全部楼层 |阅读模式
按照教程流程
https://2017-cicese-metagenomics ... atest/assemble.html


在MEGAHIT这一步报错

../megahit/megahit --12 SRR1976948.abundtrim.subset.pe.fq.gz,SRR1977249.abundtrim.subset.pe.fq.gz -o combined


--- [Thu Feb 21 09:52:10 2019] Start assembly. Number of CPU threads 80 ---
--- [Thu Feb 21 09:52:10 2019] Available memory: 270473314304, used: 243425982873
--- [Thu Feb 21 09:52:10 2019] Converting reads to binaries ---
b'    [ERROR] [read_lib_functions-inl.h : 172]: Cannot identify read library type'
b'    [ERROR] [read_lib_functions-inl.h : 173]: Valid types: pe, se, interleaved'
Error occurs when running "megahit_asm_core buildlib"; please refer to combined/log for detail
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