芯片数据处理高手教程--biostar经典帖子系列

Jimmy

原文见：https://www.biostars.org/p/53870/  是Griffith写的：

I was thinking about creating a tutorial on how to do a simple microarray analysis in Bioconductor. But, I realized this has already been done quite nicely at the Bioinformatics Knowledgeblog. Their first tutorial on the subject covers installation of necessary packages, downloading of cel files, describing the experiment, loading and normalizing data, quality controls, probe set filtering, finding differentially expressed probe sets, and finally annotating those probe sets to gene symbols. They have follow-up tutorials on visualizing microarray data with volcano plots andassessing the biological significance of results. All three seem quite excellent after a brief perusal.

I might have also included: filtering with 'genefilter', multiple testing correction with 'multtest', and visualizations with 'heatmap.2'. Let me know if you would like to see tutorials specifically on any of those topics.

UPDATE: Since this seems to be coming up with some regularity I decided to add some sample code for importing CEL files, normalizing, and annotating to Entrez Gene ID and Symbol, even though this overlaps largely with the tutorial linked above. It illustrates a few slightly different things and also gives a concrete example for the 'Affymetrix Human Gene 1.0 ST Array'.

其实就是高亮的那几个步骤，非常简单，只要你用心一步步的开始做了，就会豁然开朗~

Jimmy

这个视频教程也很棒：http://learn.genetics.utah.edu/content/labs/microarray/

果子

感谢你呀，健明！

x2yline

感谢大神分享