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[assembly] 请求解答trinity 的错误

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发表于 2016-12-23 22:31:01 | 显示全部楼层 |阅读模式
知道这个问题对网站上的大神们可能很简单,但是新手无力了,拿这个数据跑了好几次,每次都错误,求解答,万分感谢
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hread 7 terminated abnormally: Error, cannot identify first line as read name line: >SRR2062963.1/1
CTTCTGTACGCCACATTTCCCACGCCCGCCAGTCGGACGGGGATTCGGCGCTGGGCTCTTCCCTCTTCGCTCGCCGCTACTGAGGGAATCCTGGTTAGTTTCTTTTCCTCCGCTTAGTAATATGCTTAAATTCAG
>SRR2062963.2/1
ACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTCAAACTCCCCACCTGCCACTGTCCCCGGAGCGGGTCACGCCCCGCACGCGCGGGGCGCTTG
 at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/../PerlLib/Fastq_reader.pm line 97 thread 7, <> line 4.
        Fastq_record::new('Fastq_record', '>SRR2062963.1/1\x{a}CTTCTGTACGCCACATTTCCCACGCCCGCCAGTCGGACGGGGATT...') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/../PerlLib/Fastq_reader.pm line 59 thread 7
        Fastq_reader::next('Fastq_reader=HASH(0x7f8b0914b2d8)') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 507 thread 7
        main::make_normalized_reads_file('ARRAY(0x288ad18)', 'fq', 'pairs.K25.stats.C50.pctSD10000.accs', '/export/data/student/sjl/fastq/trinity_out_dir/insilico_read_...') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 411 thread 7
        eval {...} called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 411 thread 7
Error encountered with thread.
Thread 8 terminated abnormally: Error, cannot identify first line as read name line: >SRR2062963.1/2
CTGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAACTAACCAGGATTCCCTCAGTAGCGGCGAGCGAAGAGGGAAGAGCCCAGCGCCGAATCCCCGTCCGACTGGCGGGCGTGGGAAATGTGGCGTACAGAAG
>SRR2062963.2/2
CTCTAGTCTGGCACCGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCTCCGGCCGCCGGTGAAATACCACTACTCTTATCGTTTTTTCACTTACCCGGTGAGGCGGGAAGGCGAGCCCCGAGCGGGCTCT
 at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/../PerlLib/Fastq_reader.pm line 97 thread 8, <> line 4.
        Fastq_record::new('Fastq_record', '>SRR2062963.1/2\x{a}CTGAATTTAAGCATATTACTAAGCGGAGGAAAAGAAACTAACCAG...') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/../PerlLib/Fastq_reader.pm line 59 thread 8
        Fastq_reader::next('Fastq_reader=HASH(0x7f8b1514b200)') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 507 thread 8
        main::make_normalized_reads_file('ARRAY(0x293c1b8)', 'fq', 'pairs.K25.stats.C50.pctSD10000.accs', '/export/data/student/sjl/fastq/trinity_out_dir/insilico_read_...') called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 411 thread 8
        eval {...} called at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 411 thread 8
Error encountered with thread.
Error, at least one thread died at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl line 427.
Error, cmd: /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/util/insilico_read_normalization.pl --seqType fq --JM 9G  --max_cov 50 --CPU 6 --output /export/data/student/sjl/fastq/trinity_out_dir/insilico_read_normalization   --max_pct_stdev 10000  --left /export/data/student/sjl/fastq/SRR2062963_1_fixed.fastq --right /export/data/student/sjl/fastq/SRR2062963_2_fixed.fastq --pairs_together --PARALLEL_STATS   died with ret 7424 at /export/home/student/1160608013/mybiosoft/trinity/trinityrnaseq-Trinity-v2.3.2/Trinity line 2427.

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 楼主| 发表于 2016-12-24 14:27:26 | 显示全部楼层
已自己尝试各种方法解决
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